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2015 | 22 | 2 | 285-299

Article title

Bioremediation Of A Spent Metalworking Fluid With Auto- And Allochthonous Bacterial Consortia


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Spent mineral oil-based metalworking fluids are waste products of the machining processes and contribute substantially to the global industrial pollution with petroleum oil products. Wastewaters containing oily emulsions are ecologically hazardous and thus a variety of methods have been implemented to prevent these effluents from affecting the natural environment. Most of these methods rely upon physical-chemical treatment and phase separation; however, none of them proved to be effective enough to meet tightening environmental regulations. Therefore, novel technologies need to be elaborated and there is growing interest in implementing biological treatment methods based on microbial bioremediation. In this study an oil/water emulsion obtained from a waste stream of the metal-processing industry was tested for biodegradability of its organic constituents. This liquid waste was found non-toxic to bacterial consortia and was colonized with indigenous microorganisms (approx. 107 cfu · cm−3). The total load of organic content was determined as a chemical oxygen demand (COD) value of 48 200 mg O2 · dm−3. Emulsion treatment was carried out using a threefold wastewater dilution and employing two variants of biostimulated aerobic bacterial communities: (1) uninoculated emulsion, where bioremediation was carried out by the autochthonous bacteria alone, and (2) wastewater samples inoculated with a ZB-01 microbial consortium which served as a source of specialized bacteria for process bioaugmentation. Biodegradation efficiency achieved in a 14-day test was monitored by measuring both the COD parameter and the concentration of high-boiling organic compounds. Both approaches yielded satisfactory results showing significant reduction of the emulsion organic fraction; however, the resultant decrease of wastewater load tended to be more efficient for the case where the process was bioaugmented with the inoculated consortium. Gas chromatography analyses coupled with mass spectrometric detection (GC-MS) confirmed high degradation yields obtained for both cases studied (58 and 71%, respectively) in a 28-day test. It is concluded that oil-based metalworking emulsions can undergo efficient biological treatment under conditions enabling aerobic bacterial proliferation and that xenobiotic biodegradation kinetics can be accelerated by bioaugmenting the process with allochthonous microbial consortia.









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1 - 6 - 2015
19 - 9 - 2015


  • Department of Biochemistry, Institute of Plant Biology and Biotechnology, University of Agriculture in Krakow, al. 29 Listopada 54, 31-425 Kraków, Poland, phone +48 12 622 5193
  • Department of Biochemistry, Institute of Plant Biology and Biotechnology, University of Agriculture in Krakow, al. 29 Listopada 54, 31-425 Kraków, Poland, phone +48 12 622 5193
  • Department of Biochemistry, Institute of Plant Biology and Biotechnology, University of Agriculture in Krakow, al. 29 Listopada 54, 31-425 Kraków, Poland, phone +48 12 622 5193


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