Effects of mixing on the course of fast chemical reactions are relatively well understood, especially in homogeneous systems. This enables to design and operate chemical reactors with the goal to achieve a high yield of a desired product and use systems of complex reactions as a chemical probe (chemical test reactions) to identify progress of mixing and quality of mixture. Recently, a number of studies have focused on the application of chemical test reactions to identify energy efficiency of mixing, being a convenient way of comparing mixers and reactors in terms of their mixing efficiency. This review offers a presentation of chemical test reactions available in the literature and methods of applications of test reactions to identify the energy efficiency of mixing. Also methods to assess the extent of micromixing by measuring product distribution or segregation index, and to determine the time constant for mixing are presented for single phase homogeneous systems and two-phase liquid-liquid systems.