Retention behavior of selected alkaloids in Reversed Phase micellar chromatographic systems

• Authors: Anna Petruczynik , Grzegorz Noster , Monika Waksmundzka-Hajnos
• Languages of publication: EN

Abstracts

EN

In this work, the effects of sodium dodecyl sulfate (SDS) concentrations on retention, separation selectivity, peak shapes and systems efficiency were investigated. Herein, the retention data for 11 alkaloids were determined on an RP18 silica column with mobile phases containing methanol as organic modifier, with acetate buffer at pH 3.5, and, subsequently, with the addition of sodium dodecyl sulfate (SDS). The results of this study indicate that the retention of alkaloids decreases with the increase of SDS concentration in the mobile phase. The increase of SDS concentration, however, leads to the significantly improvement of peak symmetry and the increase of theoretical plate number in all cases. The best system efficiency for most of the investigated alkaloids was obtained in a mobile phase containing 0.1 M SDS, while most symmetrical peaks were obtained through the addition of 0.3 M of SDS to the mobile phase.

Keywords

EN

alkaloids; HPLC; micellar chromatography; SDS; system efficiency

• Publisher: De Gruyter Open
• Journal: Current Issues in Pharmacy and Medical Sciences
• Year: 2015
• Volume: 28
• Issue: 2
• Pages: 126-130

Dates

published

1 - 6 - 2015

online

16 - 7 - 2015

received

31 - 3 - 2015

accepted

8 - 5 - 2015

Contributors

author

Anna Petruczynik

• Department of Inorganic Chemistry Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland

author

Grzegorz Noster

• Department of Inorganic Chemistry Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland

author

Monika Waksmundzka-Hajnos

• Department of Inorganic Chemistry Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland

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Identifiers

DOI

10.1515/cipms-2015-0058