Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl


Preferences help
enabled [disable] Abstract
Number of results
2015 | 28 | 1 | 30-32

Article title

Anti-Helicobacter pylori activity in vitro of chamomile flowers, coneflower herbs, peppermint leaves and thyme herbs – a preliminary report


Title variants

Languages of publication



Recently, several studies have been undertaken so as to develop more effective therapeutic approaches towards eradicating Helicobacter pylori. Among these is phytotherapy. The aim of this study was to investigate the activity in vitro of the plant extracts obtained from common herbs cultivated in the Lubelszczyzna region against the reference strain H. pylori ATCC 43504. Among these are thyme herbs, chamomile flowers, peppermint leaves and coneflower herbs. Herein, it was found that the MIC values of the assayed extracts were as follows: the extracts from coneflower herbs showed anti-H. pylori activity with MIC = 31.3-125 μg/ml; the extracts from chamomile flowers demonstrated MIC = 31.3-62.5 μg/ ml; the extracts from peppermint leaves had MIC = 15.6-250 μg/ml; and the extracts from thyme herbs revealed MIC = 15.6-62.5 μg/ml, depending on the solvent used. The most active were the extracts obtained with ethyl acetate or ethanol alcohol absolute 99.8%. These showing MIC within the range of 15.6-62.5 μg/ml, while the lowest activity was observed in case of the extract obtained with 70% aqueous ethanol. This last showing MIC within the range of 62.5-250 μg/ml. The MIC values of essential oil components were 15.6 μg/ml for bisabolol and menthol or 31.3 μg/ml for thymol. The obtained data indicate that the assayed herbs possessed promising anti-H. pylori bioactivity.









Physical description


1 - 3 - 2015
17 - 3 - 2015
2 - 3 - 2015
9 - 5 - 2015


  • Department of Pharmaceutical Microbiology with Laboratory for Microbiological Diagnostics, Medical University of Lublin, 1 Chodzki, 20-093 Lublin, Poland
  • Department of Pharmaceutical Microbiology with Laboratory for Microbiological Diagnostics, Medical University of Lublin, 1 Chodzki, 20-093 Lublin, Poland
  • Department of Pharmaceutical Microbiology with Laboratory for Microbiological Diagnostics, Medical University of Lublin, 1 Chodzki, 20-093 Lublin, Poland
  • Department of Pharmacognosy with Medicinal Plant Unit, Medical University of Lublin, 1 Chodzki, 20-093 Lublin, Poland


  • 1. Boyanova L.: Comparative evaluation of the activity of plant infusions against Helicobacter pylori strains by three methods. World J. Microbiol. Biotechnol., 30, 1633, 2014.[WoS][Crossref]
  • 2. Dorman H.J.D, Deans S.G.: Antimicrobial agents from plants: antibacterial activity of plants volatile oils. J. Appl. Microbiol., 88, 308, 2000.[Crossref]
  • 3. Edris A.E.: Pharmaceutical and therapeutic potentials of essential oils and their individual volatile constituents: A review. Phytother. Res., 21, 308, 2007.[Crossref]
  • 4. Fadda G., Zanetti S.: In vitro activity of essential oil of Myrtus communis L. against Helicobacter pylori. Int. J. Antimicrob. Agents., 30, 562, 2007.[WoS][Crossref]
  • 5. Falsafi T. et al.: Chemical composition and anti-Helicobacter pylori effect of Satureja bachtiarica Bunge essential oil. Phytomedicine., 15, 173, 2015.[WoS][Crossref]
  • 6. French G.L.: Bactericidal agents in the treatment of MRSA infections - the potential role of daptomycin. J. Antimicrob. Chemother. 58, 1107, 2006.[Crossref]
  • 7. Hazzit M. et al.: Chemical composition and biological activities of Algerian Thymus oils. Food Chem., 116, 714, 2009.[Crossref][WoS]
  • 8. Lang G., Buchbauer G.: A review on recent research results (2008-2010) on essential oils as antimicrobial and antifungals. A review. Flavour Fragr. J., 27, 13, 2012.
  • 9. Njume C. et al.: Volatile compounds in the stem bark of Sclerocarya birrea (Anacardiaceae) possess antimicrobial activity against drugresistant strains of Helicobacter pylori. Int. J. Antimicrob. Agents., 38, 319, 2011.[Crossref]
  • 10. O`Donnell F. et al.: A study of the antimicrobial activity of selected synthetic and naturally occurring quinolines. Int. J. Antimicrob. Agents., 35, 30, 2010.[Crossref][WoS]
  • 11. Ohno T. et al.: Antimicrobial activity of essential oils against Helicobacter pylori. Helicobacter, 8, 207, 2003.[Crossref]
  • 12. Papastergiou V., Georgopoulos S.D., Karatapanis S.: Treatment of Helicobacter pylori infection: past, present and future. World J. Gastrointest. Pathophysiol., 5, 392, 2014.[Crossref]
  • 13. Rios J.L., Recio M.C.: Medicinal plants and antimicrobial activity. J. Ethnopharmacol. 100, 80, 2005.[Crossref]
  • 14. Safavi M., Shams-Ardakani M., Foroumadi A.: Medicinal plants in treatment of Helicobacter pylori infections. Pharm. Biol., 28, 1, 2014.
  • 15. Shikov A.N. et al.: Antibacterial activity of Chamomilla recutita oil extract against Helicobacter pylori. Phytoter. Res., 22, 252, 2008.[Crossref]
  • 16. Vale F.F., Oleastro M.: Overview of the phytomedicine approaches against Helicobacter pylori. World J. Gastroenterol., 20, 5594, 2014.[WoS][Crossref]
  • 17. Vitor J.M.B., Vale F.F.: Alternative therapies for Helicobacter pylori: Probiotics and phytomedicine. FEMS Immunol. Med. Microbiol., 63, 153, 2011.[WoS]
  • 18. Wiegand I., Hilpert K., Hancock R.E.W.: Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat. Protoc. 3, 163, 2008.[WoS][Crossref]

Document Type

Publication order reference


YADDA identifier

JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.