Comparative validation of HPLC, densitometric and videodensitometric determination of lamotrigine in pharmaceutical

• Authors: Beata Paw
• Languages of publication: EN

Abstracts

EN

Simple, sensitive, precise and accurate HPLC, densitometric and videodensitometric methods for determination of lamotrigine in tablet forms were developed and validated. The HPLC method was carried out using a Symmetry C8 column and a mobile phase acetonitrile-phosphate buffer pH 2.80 (25:75, v/v), with a flow rate of 1 mL/min, and UV detection at 210 nm. Ethosuximide was used as the internal standard. Densitometric and videodensitometric analysis was performed on silica gel 60 F254 plates, in horizontal chambers, with methanol-chloroform-ammonia (25%) 1.5:7.5:1, (v/v) as mobile phase. Densitometric detection was performed at 225 nm and at 315 nm, and videodeoscanning at 254 nm. Calibration plots were constructed in the range 0.5-10 μg/spot, with good correlation coefficients r > 0.99 for both methods. The precision and accuracy of all elaborated methods were compared. Finally, the developed methods were applied for the quality control of lamotrigine tablets.

Keywords

EN

densitometry; videodensitometry; lamotrigine; tablets

• Publisher: De Gruyter Open
• Journal: Current Issues in Pharmacy and Medical Sciences
• Year: 2014
• Volume: 27
• Issue: 4
• Pages: 258-262

Dates

published

1 - 12 - 2014

received

11 - 12 - 2014

accepted

15 - 12 - 2014

online

3 - 3 - 2015

Contributors

author

Beata Paw

• Chair and Department of Medicinal Chemistry, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland

References

• 1. Alizadeh N., Khakinahad R., Jabbari A.: Spectrophotometric determination of lamotrigine in pharmaceutical preparations and urine by charge-transfer complexation. Pharmazie, 63, 791, 2008.[WoS]
• 2. Bialer M. et al.: Progress report on new anti-epileptic drugs: a summary of the Eighth Eilat Conference (EILAT VIII). Epilepsy Res., 73, 1, 2007.
• 3. Calvo M.E.B., Renedo O.D., Martinez M.J.A.: Optimization of the experimental parameters in the determination of lamotrigine by adsorptive stripping voltammetry. Analytica Chimica Acta, 549, 74, 2005.
• 4. El-Enany N.M. et al.: Validated spectrofluorimetric method for the determination of lamotrigine in tablets and human plasma through derivatization with o-phthalaldehyde. J. Fluoresc. 20, 463, 2010.[Crossref][WoS]
• 5. Emami J., Ghassami N., Ahmadi F.: Development and validation of a new HPLC method for determination of lamotrigine and related compounds in tablet formulations. J. Pharm. Biomed. Anal., 40, 999, 2006.
• 6. Gupta V.K., Singh A.K., Gupta B.: Development of membrane electrodes for selective determination of some anti-epileptic drugs in pharmaceuticals, plasma and urine. Anal. Bioanal. Chem., 389, 2019, 2007.
• 7. Koba M. et al.: Determination of lamotrigine in tablets using HPTLC, HPLC, and derivative spectrophotometry methods. J. Liq. Chromatogr. & Rel. Technol., 36, 537, 2013.[WoS]
• 8. Martins M.T., Paim C.S., Steppe M.: LC and UV methods for lamotrigine determination in pharmaceutical formulation. Chromatography Research International, Article ID 860168, 2011.
• 9. Paw B., Matysiak J., Kowalczuk D.: Development and validation of a capillary electrophoresis method for the determination of lamotrigine in pharmaceutical dosage form. Ann. UMCS Sect. DDD, 24, 9, 2011.
• 10. Rajendraprasad N., Basavaiah K., Vinay K.B.: Micro and nanogram determination of lamotrigine in pharmaceuticals by visible spectrophotometry, using bromophenol blue. Indian Journal of Chemical Technology, 17, 220, 2010.
• 11. Renedo O.D, Calvo M.E.B., Martinez M.J.A.: Determination of lamotrigine in pharmaceutical preparations by adsorptive stripping volammetry, using screen printed electrodes. Sensors, 8, 4201, 2008.[Crossref]
• 12. Vinay K.B. et al.: Sensitive, selective and extraction-free spectrophotometric determination of lamotrigine in pharmaceuticals, using two sulphonthalein dyes. Thai J. Pharm. Sci., 35, 65, 2011.
• 13. Youssef N.F., Taha E.A.: Development and validation of spectrophotometric, TLC and HPLC methods for the determination of lamotrigine in the presence of its impurity. Chem. Pharm. Bull., 55, 541, 2007.[WoS]

Identifiers

DOI

10.1515/cipms-2015-0029