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2014 | 27 | 4 | 258-262
Article title

Comparative validation of HPLC, densitometric and videodensitometric determination of lamotrigine in pharmaceutical

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Content
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EN
Abstracts
EN
Simple, sensitive, precise and accurate HPLC, densitometric and videodensitometric methods for determination of lamotrigine in tablet forms were developed and validated. The HPLC method was carried out using a Symmetry C8 column and a mobile phase acetonitrile-phosphate buffer pH 2.80 (25:75, v/v), with a flow rate of 1 mL/min, and UV detection at 210 nm. Ethosuximide was used as the internal standard. Densitometric and videodensitometric analysis was performed on silica gel 60 F254 plates, in horizontal chambers, with methanol-chloroform-ammonia (25%) 1.5:7.5:1, (v/v) as mobile phase. Densitometric detection was performed at 225 nm and at 315 nm, and videodeoscanning at 254 nm. Calibration plots were constructed in the range 0.5-10 μg/spot, with good correlation coefficients r > 0.99 for both methods. The precision and accuracy of all elaborated methods were compared. Finally, the developed methods were applied for the quality control of lamotrigine tablets.
Publisher

Year
Volume
27
Issue
4
Pages
258-262
Physical description
Dates
published
1 - 12 - 2014
received
11 - 12 - 2014
accepted
15 - 12 - 2014
online
3 - 3 - 2015
Contributors
author
  • Chair and Department of Medicinal Chemistry, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland, beata.paw@umlub.pl
References
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  • 10. Rajendraprasad N., Basavaiah K., Vinay K.B.: Micro and nanogram determination of lamotrigine in pharmaceuticals by visible spectrophotometry, using bromophenol blue. Indian Journal of Chemical Technology, 17, 220, 2010.
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  • 12. Vinay K.B. et al.: Sensitive, selective and extraction-free spectrophotometric determination of lamotrigine in pharmaceuticals, using two sulphonthalein dyes. Thai J. Pharm. Sci., 35, 65, 2011.
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_1515_cipms-2015-0029
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