Preferences help
enabled [disable] Abstract
Number of results
2015 | 13 | 1 |
Article title

Influence of extraction methods on the recovery of astragaloside IV from the roots of Astragalus mongholicus in Soxhlet- and Twisselmann-type apparatus

Title variants
Languages of publication
Astragalus mongholicus, commonly used in
Traditional Chinese Medicine (TCM), contains significant
amounts of triterpene saponins – astragalosides, which
possess a wide variety of pharmacological actions,
including the treatment of cardiovascular and respiratory
diseases. The present study was performed to develop a
fast and effective extraction method of saponins from
crude plant material using Twisselmann-type apparatus.
Moreover, a detailed comparison between Soxhlet and
Twisselmann apparatus was performed to explain the
advantages and disadvantages of their applications.
The content of astragaloside IV – the major saponin of
A. mongholicus extracts and chosen as an extraction
efficiency marker ‒ was determined by means of RP-HPLCELSD
chromatography in an herein optimized method.
Twisselmann extraction shortened its duration from 4 to 2
hours in comparison with the Soxhlet method ‒ a generally
accepted method preparation for this extract. The
effectiveness of both apparatus was alike (3.24 ± 0.44 mg
and 3.48 ± 0.26 mg of astragaloside IV/ 4g of powdered
root for 2 hours in Twisselmann and 4 hours in Soxhlet
apparatus). Compared with a pharmacopoeial method, the
application of Twisselmann apparatus enabled a decrease
in analysis time of 50%, which is of high significance,
especially for large, scaled-up industrial scale.
Physical description
1 - 4 - 2015
2 - 6 - 2015
3 - 12 - 2014
  • [1] Anon. Astragalus membranaceus. Altern. Med. Rev., 2007, 8, 72-77.
  • [2] Pharmacopoeia of the People’s Republic of China, Vol. 1, People’s Medical Publishing House, Shenzen, 2001.
  • [3] Yesilada E., Bedir E., Calis I., Takahashi Y., Ohmoto Y., Effects of triterpene saponins from Astragalus species on in vitro cytokine release, J. Ethnopharmacol., 2005, 96, 71-77.[Crossref]
  • [4] Lin L.Z., He X.G., Lindenmaier M., Nolan G., Yang J., Cleary M., et al., Liquid Chromatography-Electrospray Ionization Mass Spectrometry Study of the Flavonoids of the Roots of Astragalus mongholicus and A. membranaceus, J. Chrom. A, 2000, 876, 1-2, 87-95.
  • [5] Ren S., Zhang H., Mu., Sun M., Liu P., Pharmacological effects of Astragaloside IV: a literature review, J. Tradit. Chin. Med., 2013, 15, 33, 3, 413-416.
  • [6] Hu J.Y., Han J., Chu Z.G., Song H.P., Zhang D.X., Zhang Q., et al., Astragaloside IV attenuates hypoxia-induced cardiomyocyte damage in rats by upregulating superoxide dismutase-1 levels, Clin. Exp. Pharmacol. Physiol., 2009, 36, 4, 351-357.[WoS][Crossref]
  • [7] Ji K.T., Tang J.F., Chai J.D. Effect of Astragaloside against the oxidative damage on endothelial cells. Zhong Guo Zhong Xi Yi Jie He Za Zhi 2011, 31, 6, 807-810.
  • [8] Li H.B., Gei Y.K., Zhang L., Astragaloside IV improved barrier dysfunction induced by acute high glucose in human umbiIical vein endothehal cells. Life Sci. 2006; 79, 12, 1186-1193.
  • [9] Liu X., Min W., Protective effects of Astragaloside against ultraviolet a-induced photoaging in human fibroblasts, Zhong Xi Yi Jie He Xue Bao, 2011, 9, 3, 328-332.
  • [10] Meng L.Q., Tang J.W., Wang Y., Zhao J.R., Shang M.Y.,Zhang M., et al., Astragaloside IV synergizes with ferulic acid to inhibit renal tubulointerstitial fibrosis in rats with obstructive nephropathy, Br. J. Pharmacol., 2011, 162, 8, 1805-1818.[WoS]
  • [11] Liu H., Wei W., Sun W.Y., Li X., Protective effects of Astragaloside IV on porcine-serum-induced hepatic fibrosis in rats and in vitro effects on hepatic stellate cells, J. Ethnopharmacol., 2009, 122, 3, 502-508.[WoS]
  • [12] Wang S., Li J., Huang H., Gao W., Zhuang C., Li B., et al., Anti-hepatitis B virus activities of Astragaloside IV isolated from radix Astragali, Biol. Pharm. Bull., 2009, 32, 1, 132-135.[Crossref][WoS]
  • [13] European Pharmacopoeia 8.0 edition, 2013.[PubMed]
  • [14] Monschein M., Ardjomand-Woelkart K., Rieder J., Wolf I., Heydel B., Kunert O., et. al., Accelerated sample preparation and formation of astragaloside IV in Astragali radix, Pharm Biol. (In press), DOI: 10.3109/13880209.2013.839712.[Crossref]
  • [15] Jalsrai A., Grecksch G., Becker A., Evaluation of the effects of Astragalus mongholicus Bunge saponin extract on central nervous system functions, J. Ethnopharmacol., 2010, 131, 3, 544-549.[WoS]
  • [16] Kloeck G., Noke A., Christiansen T., Soxhlet or Twisselmann, Filtr. Separat., 2013, 33, 32-33.
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.