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2015 | 13 | 1 |
Article title

Application of QuEChERS - High Performance
Liquid Chromatography with Postcolumn
Fluorescence Derivatization (HPLC-FLD) method
to analyze Eprinomectin B1a residues from
a pour-on conditioning in bovine edible tissues

Title variants
Languages of publication
A QuEChERS in house method for determining
the marker residue of eprinomectin (eprinomectin B1a)
by HPLC-FLD in bovine tissues and milk provided from
treated animals was developed and applied. Briefly: all
samples were extracted with acetonitrile using a dispersive
SPE purification stage. The ascertained detection limits
were 1 μg kg-1 and the quantification limits 2 μg kg-1.
Recoveries on tissue samples fortified in the range of
10 μg kg-1 to 200 μg kg-1 were from 80.0% to 87.2%, with
variation coefficients between 2.7% to 10.6%. The
confirmation of residues in the purified extracts was
made by LC-MS/MS after separation on an XTerra MS C18
(10 cm × 2.1 mm, 3.5 μm) column with a mobile phase of
acetonitrile / formic acid 0.1% (97:3, v/v) at a flow rate of
0.2 mL min-1 and MRM monitoring of three characteristic
ions (m/z 896.1, m/z 467.9 and m/z 329.9), resulting from
the fragmentation of molecular ions [M-H]+ (m/z 914.6)
of eprinomectin and the comparison of the abundance ratio of fragmented ions was obtained in the booth,
sample and standard at comparative concentrations.
In conclusion, this method has proven its advantage and
versatility as a routine drug residues analysis method.
Physical description
12 - 2 - 2015
16 - 1 - 2015
29 - 10 - 2014
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