This report describes the high yield
biotransformation of 1,8-cineole by the strain Gymnopilus
spectabilis 7423, a common fungus isolated from the
Eucalyptus tree. The biotransformation was conducted
under resting cell conditions and different parameters
were tested in order to achieve up to 90% bioconversion.
Only two regioisomers were detected, and they were
identified as 3-α-hydroxy-1,8-cineole and 2-α-hydroxy-1,8-
cineole obtained in a 82:8 ratio.