The present study aimed to investigate the
relationship between semen quality parameters and
DNA integrity, and determine whether semen quality
parameters could serve as a reliable biomarker for
monitoring sperm DNA damage. Conventional semen
parameters from a total of 202 male human subjects were
analyzed. DNA fragmentation and 8-oxo-7,8-dihydro-2′-
deoxyguanosine (8-oxoGuo) were used to assess sperm
DNA integrity. DNA fragmentation was analyzed by the
terminal deoxynucleotidyl transferase dUTP nick end
labeling (TUNEL) assay and sperm chromatin structure
assay (SCSA), while 8-oxodGuo was quantified by the
liquid chromatography/tandem mass spectrometry
(LC-MS/MS) coupled with an on-line solid phase system.
The levels of 8-oxodGuo levels in sperm were related to the
percentages of DNA fragmentation measured by both the
TUNEL and SCSA (r = 0.22, p = 0.048; r = 0.12, p = 0.039).
Sperm vitality, motility and morphology from all of the
participants exhibited a weak correlation with the levels
of 8-oxodGuo and the percentages of DNA fragmentation.
Semen quality parameters may be independent of the
formation of DNA fragmentation and oxidative adducts in
sperm. Semen quality parameters may be insufficient to
monitor sperm DNA fragmentation and oxidative damage.
DNA damage in sperm is recommended to be included in