Angiogenic Effect of Bioactive Borate Glass
Microfibers and Beads in the Hairless Mouse

• Authors: Richard J. Watters , Roger F. Brown , Delbert E. Day
• Languages of publication: EN

Abstracts

EN

The purpose of this project was to investigate
the angiogenic mechanism of bioactive borate glass for
soft tissue repair in a ‘hairless’ SKH1 mouse model. Subcutaneous
microvascular responses to bioactive glass microfibers
(45S5, 13-93B3, and 13-93B3Cu) and bioactive
glass beads (13-93, 13-93B3, and 13-93B3Cu) were assessed
via: noninvasive imaging of skin microvasculature; histomorphometry
of microvascular densities; and quantitative
PCR measurements of mRNA expression of VEGF
and FGF-2 cytokines. Live imaging via dorsal skin windows
showed the formation at twoweeks of a halo-like structure
infused with microvessels surrounding implanted boratebased
13-93B3 and 13-93B3Cu glass beads, a response not
observed with silicate-based 13-93 glass beads. Quantitative
histomorphometry of tissues implanted with plugs of
45S5, 13-93B3, and 13-93B3Cu glass microfibers revealed
microvascular densities that were 1.6-, 2.3-, and 2.7-times
higher, respectively, than the sham control valueswhereas
13-93, 13-93B3, and 13-93B3Cu glass beads caused the microvascular
density to increase 1.3-, 1.6-, and 2.5-fold,
respectively, relative to sham controls. Quantitative PCR
measurements indicate a marginally significant increased
expression of VEGF mRNA in tissues with 13-93B3Cu glass
beads, an outcome that supported the hypothesis that
copper-doped borate glass could promote VEGF expression
followed by angiogenesis for enhanced wound healing.

Keywords

EN

Angiogenesis; copper-doped bioactive borateglass; bioactive glass microfibers; bioactive glass beads; invivo; soft tissue

• Publisher: De Gruyter Open
• Journal: Biomedical glasses
• Year: 2015
• Volume: 1
• Issue: 1

Dates

received

15 - 10 - 2015

online

24 - 12 - 2015

accepted

5 - 12 - 2015

Contributors

author

Richard J. Watters

• Department of Biological Sciences and
  2Graduate Center for Materials Research, Missouri University of
  Science and Technology, Rolla, MO 65401

author

Roger F. Brown

• Department of Biological Sciences and
  2Graduate Center for Materials Research, Missouri University of
  Science and Technology, Rolla, MO 65401

author

Delbert E. Day

• Graduate Center for Materials Research Missouri
  University of Science and Technology

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Identifiers

DOI

10.1515/bglass-2015-0017