Objectives. The aim was to introduce a new
methodology to characterize toothpaste containing bioactive
glass and to evaluate the effect of release of fluoride
ions, by cleaving monofluorophosphate (MFP), on the
mineral forming ability of Sensodyne Repair & Protect
(SRP). which contains NovaMinTM (bioactive glass, 45S5
composition). Methods. SRP, NovaMin particles, and placebo paste (PLA)
which did not contain NovaMin, were immersed into
a remineralization media (RS), which mimics the ionic
strength of human saliva, for 3 days with different concentrations
of alkaline phosphatase (ALP): 0, 25 and 75 U.L−1.
Ion concentration profiles and pH were monitored by ICPOES
and F− ion selective electrode. Remaining solids were
collected by freeze-drying and their surfaces analysed. Results. Hydroxyapatite (HA) formed on the surface of
BG alone (after 1 h) and in toothpaste (after 2 h), whereas
PLA did not induce any precipitation. ALP cleaved MFP at
different rates depending on the enzyme concentration.
Increasing the concentration of ALP from 0 and 75 U.L−1 reduced the time of HA formation from 2 h to 24 h. However,
the presence of fluoride induced the precipitation of
fluorapatite. No evidence of fluorite (CaF2) was observed.
The apatite formation ability of toothpaste can be assessed
using the presented method.