AFM study of complement system assembly initiated by antigen-antibody complex

• Authors: Almira Ramanaviciene , Valentinas Snitka , Rasa Mieliauskiene , Rolandas Kazlauskas , Arunas Ramanavicius
• Languages of publication: EN

Abstracts

EN

The shape and size of complement system C1 components assembled on a SiO2 surface after classical activation by antigen-antibody complex was determined by tapping mode atomic force microscopy (AFM). The SiO2 substrate was silanized and bovine leukemia virus proteins gp51 were covalently bound to the SiO2 substrate. Self-assembly of complement system proteins was investigated by AFM. Uniform coating of silanized surface by gp51 proteins was observed by AFM. After incubation of gp51 coated substrate in anti-gp51 antibody containing solution, Ag-Ab complexes were detected on the substrate surface by AFM. Then after treatment of Ag-Ab complex modified substrate by guinea-pig blood serum containing highly active complement system proteins for 3 minutes and 30 minutes features 2–3 times and 5–8 times higher in diameter and in height if compared with those observed after formation of Ag-Ab complex, were observed respectively on the surface of SiO2. This study revealed that AFM might be applied for the imaging of complement system assembly and provides valuable information that can be used to complement other well-established techniques.

Keywords

EN

Bionanotechnology; antigen-antibody docking; complement system; bovine leukemia virus ; AFM

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2006
• Volume: 4
• Issue: 1
• Pages: 194-206

Dates

published

1 - 3 - 2006

online

1 - 3 - 2006

Contributors

author

Almira Ramanaviciene

author

Valentinas Snitka

• Research Center for Microsystems and Nanotechnology, Kaunas University of Technology, Studentu 65, 3031, Kaunas, Lithuania

author

Rasa Mieliauskiene

• Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius 09, Lithuania

author

Rolandas Kazlauskas

• Department of Analytical and Environmental Chemistry, Vilnius University, Naugarduko 24, 03225, Vilnius 09, Lithuania

author

Arunas Ramanavicius

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Identifiers

DOI

10.1007/s11532-005-0015-8